The aim of the study: HPV16 and HPV18 E6, E7 genes expression assessment in tissues during surgical from patients with vulvar carcinoma. Material and methods: The material was 121 tissue samples obtained during oncological surgery in patients with vulvar carcinoma, which were distinguished on histopathologic examination as vulvar carcinoma (group A), metastases of vulvar carcinoma in lymphatic nodes (group B), no metastases in lymphatic nodes (group C), tissues without neoplastic lesions (group D). Further analysis was performed with QRT-PCR (TaqMan). Results: E6 HPV16 gene was highly expressed in all examined samples. E7 HPV16 was increasingly expressed only in vulvar tissues with neoplastic lesions and in lymphatic nodes with metastatic lesions. E7 HPV18 gene expression was not increased in vulvar carcinoma tissues and its expression was increased only in the lymphatic nodes without metastatic changes. Conclusions: There is a reverse expression of HPV genes in lymphatic nodes compared to the tissues of vulva, with a definite predominance of HPV18 in nodes without metastatic lesions and HPV16 in nodes with metastases, contrary to the tissues of vulva, where the predominancy of HPV16 is found in the tissues without neoplastic lesions, and HPV18 in vulvar carcinoma, which indicates worse predictions in patients with HPV16 infections.